Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division

نویسندگان

  • Gregory C. Rogers
  • Kitty K. Chui
  • Edwin W. Lee
  • Karen P. Wedaman
  • David J. Sharp
  • Gina Holland
  • Robert L. Morris
  • Jonathan M. Scholey
چکیده

We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP 180 , in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP 180 concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP 180 is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

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A Kinesin-Related Protein, Krp180, Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division

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تاریخ انتشار 2000